RPPA pioneers and other internationally recognized scientists will communicate the technology’s applications for making advancements in personalized medicine; identifying therapeutic targets; discovering and validating biomarkers; and answering high impact biological and medical questions. Attendees will also have the opportunity to attend hands-on workshops, poster sessions, complimentary coffee breaks, lunches, and a networking dinner.
The hands-on workshops will demonstrate technologies for up-stream sample preparation as well as printing, staining and analyzing Reverse Phase Protein Arrays. We will be using technologies available at George Mason University. Technologies may vary depending on individual lab equipment and preferences.
We invite 2015 Global RPPA Workshop attendees to sign-up to attend hands-on workshops scheduled on October 12-13, 2015 from 1:15pm-2:15pm. Please sign-up in advance using the electronic form below. Opportunities are limited and considered on a first come, first serve basis. Preference is given to currently registered attendees. Participation in hands-on workshops is free of charge for registered attendees.
Laser Capture Microdissection
Users will learn to isolate a pure population of cells under direct microscopic visualization using Laser Capture Microdissection (LCM). LCM can be used to collect specific cells from a heterogeneous tissue section or cell culture. The cells may be used for downstream analysis of DNA, RNA or proteins. (30 minutes)
Printing and Staining Reverse Phase Protein Arrays
Users will learn to print and stain Reverse Phase Protein Arrays, which are used to quantitatively measure post-translationally modified proteins (e.g. phosphorylated or cleaved), or total (phosphorylated and non-phosphorylated) proteins from a limited amount of sample. This platform can be used to evaluate tissue samples, cells, serum and body fluids. (1 hour)
Reverse Phase Protein Array Raw Data Post Processing
Users will learn to use George Mason University’s custom excel macro, Reverse-Phase Protein Microarray Analysis Suite (RAS), to simplify data post-processing. Following the acquisition of raw data from spot analysis software, RAS will guide users through the process of data quality control, negative control subtraction, and normalization. (30 minutes)
Grace Bio-Labs ArrayCAM Image Capture and Data Analysis
Users will learn to use the ArrayCAM microarray imager to sequentially identify multiple fluorescent endpoints on the same microarray. The software tutorial will include the selection of imaging parameters and optimization, creating automated spot finding templates, image processing, and exporting data for analysis. (30 minutes)
Nanoparticle Sample Preparation
Users will learn to use nanoparticles to trap, concentrate and protect low molecular weight proteins from body fluids such as blood, urine and saliva samples, in one step. The hydrogel nanoparticles concentrate biomarkers of interest, which can be eluted from the nanoparticle and measured using standard platforms such as Reverse Phase Protein Microarrays, Mass Spectrometry, ELISA, and Western Blotting. (1 hour)