Reverse Phase Protein Microarray Analysis Suite
The Reverse-Phase Protein Microarray Analysis Suite (RAS) is an excel macro that simplifies data post-processing after the acquisition of raw data from spot analysis software. It will, for example, remove flagged spots from downstream analysis, filter data by CV and background cutoff values, match each data point with the appropriate negative control and normalize either by single normalizer or the geometric mean of several normalizers.
Choose your preferred cv-cutoff, background-cutoff, number of replicates, etc.
Flexibility to select appropriate negative control and normalizer per endpoint
Retrieve your filtered, negative subtracted, normalized results